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PUNIT KOHLI
Associate Professor
B. Tech., Indian Institute of Technology
M. Appl. Sc., University of South Australia
Ph.D., Michigan State University
Postdoctoral Associate, University of Florida
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Research Interests
We focus on the design, synthesis, characterization and applications of nanoscale materials functionalized with (bio)chemical molecules/polymers. We are particularly interested in the fabrication of biosensors based on fluorescence resonance energy transfer mechanism;self- and forced assembly of nanomaterials; template-synthesis of novel nanotubes and nanowires; and controlling and probing nanoscale environments inside of nanotubes. We synthesized nanomaterials using template-synthesis method; the materials synthesized takes the shape, size and morphology of the template used. This is a very general and versatile method for preparing nanoscale materials. Using this method, we have synthesized nanotubes and nanowires of various shapes (conical, cylindercal,bent, titled); sizes (with inner diameter of nanotubes randing from <1 nm to > 5 micron); and materials (silver, gold,copper, polymers, silica etc.). We modify the surface of nanoparticles with nucleic acids, proteins, biocompatible polymers etc. This imparts them with surface functionalities that are useful in many applications such as biosensing, bioseparations, and gene and drug delivery etc. The characterization of nanomaterials is carried out using electron microscopy (SEM and TEM), AFM,optical spectroscopy and microscopy (UV-Vis, Fluorescence, and FTIR), surface analysis (XPS, ellipsometry, and SIMS).
Financial support from NSF, NIH, ORDA and MTC are gratefully acknowledged. |
Selected Publications
Ho, Andrea M.; Rim, Jee; Kohli, Punit; Patankar, Neelesh; Espinosa, Horacio D. “Electric-field Induced Direct Delivery of Proteins by a Nanofountain Probe” PNAS (submitted).
Afzal, Ahmed J.; Kohli, Punit; Lightfoot, David A. “Plant receptor-like serine-threonine kinases: Common elements in the interactome underlying signaling and plant defense” Current trends in Plant Science (submitted).
Li, Xuelian; Matthews, Shelton; Kohli, Punit “Fluorescence Resonance Energy Transfer in Polydiacetylene Liposomes” J. Phys. Chem. B (in press).
Gatebe, Erastus; Herron, Hayley; Zakeri, Rashid; Pradeep, R. R.; Aouadi, Samir; Kohli, Punit “Synthesis and characterization of polydiacetylene films and nanotubes” Langmuir (in press).
Basnyat, P.; Luster, B.; Muratore, C.; Voevodin, A.A.; Haasch, R.; Zakeri, R.; Kohli, P.; Aouadi, S.M. “Surface Texturing for Adaptive Solid Lubrication” Surface & Coatings Technology 2008, doi: 10.1016/j.surfcoat.2008.07.033.
Aouadi, Samir M.; Paudel, Yadab; Luster, Brandon; Stadler, Shane; Kohli, Punit; Muratore, Christopher; Hager, Carl; Voevodin, Andrey A. “Adaptive Mo2N/MoS2/Ag Tribological Nanocomposite Coatings for Aerospace Applications” Tribology Letters 2008, 29, 95–103.
Zakeri, Rashid; Clay, Watts ; Wang, Haibo; Kohli, Punit “Synthesis and Characterization of Non-linear Nanopores in Alumina Films“ Chemistry of Materials 2007, 19, 1954-1963.
Buyukserin, Fatih; Kohli, Punit; Wirtz, Mark O.; Martin, Charles R. “Electroactive Nanotube Membranes and Redox-Gating“ Small 2007, 3, 266-270.
Li, Xuelian; McCarroll, Matthew; Kohli, Punit “Modulating Fluorescence Resonance Energy Transfer in Conjugated Liposomes“ Langmuir 2006, 22, 8615-8617.
Kertzman, Z.; Aul, A.; Marchal, J.; Filip, P.; Kohli, P.; Aouadi, S. M. “Mechanical, Tribological, and Biocompatibility Properties of ZrN-Ag Nanocomposite Films“, J. Biomed. Mat. Res. (in press).
Kohli, P.; Harrell, C. C.; Zehui,C.; Gasparac, R.; Tan, W.; Martin, C. R. "DNA-functionalized Nanotube Membranes for Single-Mismatch DNA Selectivity" Science, 2004, 305, 984-986.
Harrell, C.C.; Kohli, P.; Siwy, Z.; Martin, C. R. "DNA-Nanotube Artificial Ion Channels" J. Am. Chem. Soc., 2004, 126, 15646-15647.
Martin, C. R.; Kohli, P. "The Emerging Field of Nanotube Biotechnology" Nature Drug Discovery, 2003,
2, 29.
Siwy, Z.; Heins, E.; Harrell, C. C.; Kohli, P.; Martin, C.
R. "Conical-Nanotube Voltage-Gated Ion Channel Mimics-The
Role of Surface Charge" J. Am. Chem. Soc., 2004,
126, 10850-10851.
Hou, S.; Harrell, C. C.; Trofin, L. D.; Kohli, P.; Martin,
C. R. "Layer-by-layer Strategy for formational of hybrid
Zr-Organophonate Nanotubes" J. Am. Chem. Soc.,
2004, 126, 5674-5675.
Gasparac, R.; Kohli, P.; Pauliono, M.O.M.; Trofin, L.D..;
Martin, C. R. "Template Synthesis of Nano Test Tubes", Nano Letters, 2004, 4, 513-516.
Kohli, P.; Wharton, J. E.; Baride, O.; Martin, C. R. "Template
Synthesis of Gold Nanotubes in an Anodic Alumina Membrane" J. Nanoscience and Nanotechnology, 2004, 4,
605-610.
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