Preparation of Sample:
The UV-Vis spectrophotometer is a sensitive instrument. Your sample must be dilute to obtain a proper spectrum. If your sample is a liquid, place one drop of your sample in a small test tube, and fill it full with HPLC grade methanol. If your sample is a solid, place 1-2 crystals in a small test tube, and fill it full with HPLC grade methanol.
Obtaining a Spectrum on the Genesys 10:
1.) Turn ON switch on back of instrument and wait for warm up and calibration self/check procedure. When ³Scanning² menu comes on screen‹(Test name UV scan<saved>)
Press green button below ³RUN TEST² at bottom of screen
2.) Obtain a quartz cuvette from your T.A. Fill the quartz cuvette full with HPLC grade methanol, and place the sample cuvette in the B position of sample turret. Make sure the cuvette is clean. (Inspect for fingerprints and filth.)
3.) Press green button below ³Collect Baseline²
4.) After baseline is collected, remove reference/blank sample and insert your sample in position 1 in sample turret. Press 1 on the turret diagram to move turret in position to read sample 1
5.) Press green button below ³Measure Sample²
If no new baseline is needed, load next sample in turret 1 position and Press ³Measure Sample²
If new baseline is needed, insert reference/blank in B position of turret, press B on turret diagram to move turret to B position and press button below ³Collect Basline²
Each run will print automatically when sample run is completed.
Generally we only need to collect data from about 215
400 nm since our unknowns are not highly colored in the visible region of the
spectrum.